Plasmid

Part:BBa_K4113028:Design

Designed by: Wenhui Ren   Group: iGEM22_FAFU-China   (2022-10-10)


pCAMBIA1301SN plasmid


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2556
    Illegal NheI site found at 6000
    Illegal NotI site found at 5407
    Illegal NotI site found at 6697
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 11588
    Illegal XhoI site found at 9443
    Illegal XhoI site found at 10537
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3320
    Illegal NgoMIV site found at 3494
    Illegal NgoMIV site found at 3544
    Illegal NgoMIV site found at 3548
    Illegal NgoMIV site found at 3575
    Illegal NgoMIV site found at 4074
    Illegal NgoMIV site found at 4432
    Illegal AgeI site found at 6494
    Illegal AgeI site found at 10164
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 6397
    Illegal SapI site found at 7088
    Illegal SapI.rc site found at 6625


Design Notes

Focus on the accuracy of the target gene and the enzyme cut site



Source

FAFU-China experimental group


References